Welcome to Cardiology Today – Recorded December 05, 2025. This episode summarizes 5 key cardiology studies on topics like diagnosis and formalin-fixed paraffin-embedded. Key takeaway: First FFPE Heart Rejection Molecular Test Validated.
Article Links:
Article 1: Safety, Utility, and Outcomes of Procainamide Challenge for the Diagnosis and Exclusion of Brugada Syndrome. (Circulation)
Article 2: Heart allograft rejection: molecular diagnosis using intra-graft targeted gene expression profiling. (European heart journal)
Article 3: Oestrogen-related receptor γ in sepsis-induced cardiomyopathy: role of cardiomyocyte subtype conversion. (European heart journal)
Article 4: Cardiovascular disease in women: traditional and sex-specific risk factors. (European heart journal)
Article 5: Impact of left ventricular assist device implantation on right ventricular-pulmonary arterial coupling assessed by high-fidelity hemodynamics. (The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation)
Full episode page: https://podcast.explainheart.com/podcast/first-ffpe-heart-rejection-molecular-test-validated-12-05-25/
Featured Articles
Article 1: Safety, Utility, and Outcomes of Procainamide Challenge for the Diagnosis and Exclusion of Brugada Syndrome.
Journal: Circulation
PubMed Link: https://pubmed.ncbi.nlm.nih.gov/41342099
Summary: This study characterized the safety and diagnostic yield of procainamide infusion for inducing a type one Brugada pattern for Brugada syndrome diagnosis. It established the prognosis associated with a procainamide-induced Brugada pattern, demonstrating differences from other sodium channel blockers. The investigation defined the specific indications for procainamide challenge using data from the Canadian Hearts in Rhythm Organization registry. This established a clearer clinical utility for identifying this particular electrocardiogram pattern.
Article 2: Heart allograft rejection: molecular diagnosis using intra-graft targeted gene expression profiling.
Journal: European heart journal
PubMed Link: https://pubmed.ncbi.nlm.nih.gov/41342627
Summary: This study developed and validated the first formalin-fixed, paraffin-embedded tissue-based molecular diagnostic system for heart transplant rejection. The system utilizes intra-graft targeted gene expression profiling to refine diagnostic accuracy. This method is applicable to endomyocardial biopsies and is designed for easy implementation in clinical practice. The international study established a deeply phenotyped cohort of heart transplant recipients to support this validation.
Article 3: Oestrogen-related receptor γ in sepsis-induced cardiomyopathy: role of cardiomyocyte subtype conversion.
Journal: European heart journal
PubMed Link: https://pubmed.ncbi.nlm.nih.gov/41342227
Summary: This study identified oestrogen-related receptor gamma as a key regulator in sepsis-induced cardiomyopathy. Researchers found that cardiomyocyte subtype conversion plays a crucial role in the pathophysiology of sepsis-induced cardiomyopathy. This mechanistic insight offers a novel understanding of how sepsis increases overall mortality through cardiac dysfunction. The findings lay a foundation for developing targeted therapies for sepsis-induced cardiomyopathy, addressing a current lack of specific treatments.
Article 4: Cardiovascular disease in women: traditional and sex-specific risk factors.
Journal: European heart journal
PubMed Link: https://pubmed.ncbi.nlm.nih.gov/41342194
Summary: This review highlighted that cardiovascular disease accounts for more deaths in women than breast cancer, lung cancer, and chronic lung disease combined, showing comparable mortality to men. It identified a critical lack of awareness among both women and physicians regarding cardiovascular disease as a major cause of morbidity and mortality in women. This contributes to significant delays in diagnosis and treatment. The analysis confirmed insufficient evidence often impedes effective, life-saving care for women with cardiovascular disease, despite advances in diagnosis and treatment.
Article 5: Impact of left ventricular assist device implantation on right ventricular-pulmonary arterial coupling assessed by high-fidelity hemodynamics.
Journal: The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation
PubMed Link: https://pubmed.ncbi.nlm.nih.gov/41344641
Summary: This study characterized intraoperative right ventricular adaptations following left ventricular assist device implantation. The research demonstrated how right ventricular-pulmonary artery coupling, defined as the ratio of end-systolic to arterial elastance, reflects right ventricular performance under altered loading conditions. The investigation, which used high-fidelity hemodynamics with right ventricular conductance catheters and three-dimensional echocardiography, provided specific measurements of these adaptations. This prospective observational study established a framework for understanding right ventricular response to left ventricular assist device support.
Transcript
Today’s date is December 05, 2025. Welcome to Cardiology Today. Here are the latest research findings.
Article number one. Safety, Utility, and Outcomes of Procainamide Challenge for the Diagnosis and Exclusion of Brugada Syndrome. This study characterized the safety and diagnostic yield of procainamide infusion for inducing a type one Brugada pattern for Brugada syndrome diagnosis. It established the prognosis associated with a procainamide-induced Brugada pattern, demonstrating differences from other sodium channel blockers. The investigation defined the specific indications for procainamide challenge using data from the Canadian Hearts in Rhythm Organization registry. This established a clearer clinical utility for identifying this particular electrocardiogram pattern.
Article number two. Heart allograft rejection: molecular diagnosis using intra-graft targeted gene expression profiling. This study developed and validated the first formalin-fixed, paraffin-embedded tissue-based molecular diagnostic system for heart transplant rejection. The system utilizes intra-graft targeted gene expression profiling to refine diagnostic accuracy. This method is applicable to endomyocardial biopsies and is designed for easy implementation in clinical practice. The international study established a deeply phenotyped cohort of heart transplant recipients to support this validation.
Article number three. Oestrogen-related receptor γ in sepsis-induced cardiomyopathy: role of cardiomyocyte subtype conversion. This study identified oestrogen-related receptor gamma as a key regulator in sepsis-induced cardiomyopathy. Researchers found that cardiomyocyte subtype conversion plays a crucial role in the pathophysiology of sepsis-induced cardiomyopathy. This mechanistic insight offers a novel understanding of how sepsis increases overall mortality through cardiac dysfunction. The findings lay a foundation for developing targeted therapies for sepsis-induced cardiomyopathy, addressing a current lack of specific treatments.
Article number four. Cardiovascular disease in women: traditional and sex-specific risk factors. This review highlighted that cardiovascular disease accounts for more deaths in women than breast cancer, lung cancer, and chronic lung disease combined, showing comparable mortality to men. It identified a critical lack of awareness among both women and physicians regarding cardiovascular disease as a major cause of morbidity and mortality in women. This contributes to significant delays in diagnosis and treatment. The analysis confirmed insufficient evidence often impedes effective, life-saving care for women with cardiovascular disease, despite advances in diagnosis and treatment.
Article number five. Impact of left ventricular assist device implantation on right ventricular-pulmonary arterial coupling assessed by high-fidelity hemodynamics. This study characterized intraoperative right ventricular adaptations following left ventricular assist device implantation. The research demonstrated how right ventricular-pulmonary artery coupling, defined as the ratio of end-systolic to arterial elastance, reflects right ventricular performance under altered loading conditions. The investigation, which used high-fidelity hemodynamics with right ventricular conductance catheters and three-dimensional echocardiography, provided specific measurements of these adaptations. This prospective observational study established a framework for understanding right ventricular response to left ventricular assist device support.
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Keywords
diagnosis, formalin-fixed paraffin-embedded, oestrogen-related receptor gamma, molecular diagnosis, diagnosis delays, treatment gaps, sodium channel blocker, hemodynamics, endomyocardial biopsy, Heart transplant rejection, pulmonary arterial coupling, Brugada syndrome, gene expression profiling, right ventricular adaptation, pathophysiology, cardiomyocyte subtype conversion, electrocardiogram pattern, end-systolic to arterial elastance, sepsis, Left ventricular assist device, Procainamide, Cardiovascular disease in women, Sepsis-induced cardiomyopathy, sex-specific risk factors, mortality.
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